- Jun 14, 2022
- 1 min read
Updated: Jun 27, 2022
Phospholipase C: IP3 - DAG Pathway
Seen downstream of Gq Protein-coupled receptors.
Gq protein is composed of 3 subunits: α, β, and γ.
Resting Condition
Trimer of α, β, and γ subunit is associated with the receptor.
α subunit is bound to GDP.
Activation and Signaling
Formation of IP3 and DAG
Ligand binds with the receptor
↓
Conformational change
↓
Release of GDP and binding of GTP to α subunit
↓
Dissociation of the complex from receptor & separation of subunits
↓
Formation of free α subunit and βγ complex
↓
α subunit travels along the membrane
↓
Goes to a membrane-bound enzyme, phospholipase C
↓
α subunit activates phospholipase C
↓
Phospholipase C breaks down a membrane phospholipid, PIP2 (phosphatidylinositol 4,5 bis-phosphate)
↓
Formation of:
IP3 (inositol 1,4,5-tris-phosphate) &
DAG (diacylglycerol)
Effects downstream of IP3
IP3 is water-soluble
↓
Travels through the cytosol
↓
Binds with calcium channel on endoplasmic reticulum
↓
Opening of the channel
↓
Release of calcium from the endoplasmic reticulum into the cytosol
↓
Calcium binds with various calcium-binding proteins for example calmodulin
↓
Formation of Calcium-Calmodulin complex
↓
Modulates the activity of Calcium-Calmodulin dependent kinases (CaM kinases) for example activation of Myosin Light Chain Kinase (MLCK) in smooth muscle cell
↓
Smooth muscle contraction
Other effects of increased calcium level:
Glandular secretion.
Transmitter release.
Eicosanoid synthesis.
Cell proliferation, etc.
Effects downstream of DAG
DAG
↓
Activates Protein Kinase C (PKC)
↓
PKC Phosphorylates various proteins and modulates their activity.
↓
Different responses in different cells
For example:
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) proteins contribute to changing cell shape.
Transcription factors affect gene expression.
Termination of the Signal
α subunit
↓
has GTPase activity
↓
Hydrolyses GTP into GDP and inorganic phosphate
↓
Inactive α subunit
↓
Reassociates with βγ complex
IP3
↓
Dephosphorylated
↓
Formation of inositol
↓
Reutilized in the synthesis of PIP2
DAG
↓
Converted to phospholipids
Calcium
↓
Pumped back into the endoplasmic reticulum or out of the cell
↓
Downstream activity decreases
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